圈門策略：

小鼠心臟細胞流式分選或分析需根據(jù)細胞類型、實驗目的選擇合適的圈門策略。以下是關鍵要點和方案。

巨噬細胞圈門策略：

?表面標志物選擇?：CD11b（單核/巨噬細胞標志）結合F4/80（巨噬細胞特異性抗原）可有效區(qū)分巨噬細胞群體。 ?

?散射參數(shù)?：前向散射（FSC）和側向散射（SSC）可輔助篩選細胞大小和復雜度，排除雜質細胞。 ?

?補償調(diào)節(jié)?：需對FITC、PE等熒光染料進行補償設置，避免信號溢漏導致假陽性。 ?

論文信息：

論文題目：Cardiac macrophages prevent sudden death during heart stress

期刊名稱：Nature Communications

時間期卷：12, Article number: 1910 (2021)

在線時間：2021年3月26日

DOI：doi.org/10.1038/s41467-021-22178-0

產(chǎn)品信息：

貨號：CP-005-005

規(guī)格：5ml+5ml

品牌：Liposoma

產(chǎn)地：荷蘭

名稱：Clodronate Liposomes& Control liposomes

辦事處：Target Technology(靶點科技)

Clodronate Liposomes氯膦酸鹽脂質體清除肝臟和腫瘤巨噬細胞，疾病模型為：肺動脈環(huán)縮術(pulmonary artery banding, PAB)模型。Pulmonary artery banding (PAB) 是通過環(huán)束帶限制肺動脈血流的姑息性手術，主要用于治療先天性心臟病，如室間隔缺損、大動脈轉位等伴隨過度肺血流的疾病。 ?原理: 該手術通過聚四氟乙烯帶環(huán)束肺動脈主干，降低肺動脈壓力和血流量，緩解心臟負擔。早期作為復雜先心病分期治療的過渡手段，現(xiàn)僅用于多發(fā)性室間隔缺損、合并主動脈縮窄等特殊病例。荷蘭Liposoma巨噬細胞清除劑Clodronate Liposomes見刊于Nature Communications：心臟巨噬細胞在心臟壓力期間防止猝死。

Liposoma巨噬細胞清除劑Clodronate Liposomes氯膦酸二鈉脂質體的材料和方法文字描述：

Animal studies

Male C57BL6/J mice were purchased from CLEA Japan and Jackson Laboratory and maintained on a standard mouse chow diet under sterile barrier conditions, on a 12-h light-dark cycle with 18–23?°C and 40–60% humidity. Male Areg homozygous null (Areg?/?) mice were backcrossed to C57BL6/J mice over ten generations. Rag2?/?, Cd4?/?, and Cd8a?/? mice were purchased from Taconic (Germantown, NY). The genotypes of all mice were determined using genomic PCR. The isoproterenol challenge entailed bolus administration of isoproterenol (Sigma) 5?mg/kg intraperitoneally under constant ECG recording. Clodronate liposomes and control liposomes were purchased from LIPOSOMA B.V. A total of 10?μl of clodronate or control liposome solution per gram of mouse were intravenously administrated 24?h before PAB. After the first administration of clodronate or control liposomes, the same volume of liposomes was also administrated every 7 days for long term depletion, per the manufacturer’s instructions. All protocols for animal experiments were approved by the Animal Care and Use Committee of the University of Tokyo.

Liposoma巨噬細胞清除劑Clodronate Liposomes氯膦酸二鈉脂質體的材料和方法文獻截圖：