論文信息：

論文題目： AMFR drives allergic asthma development by promoting alveolar macrophage–derived GM-CSF production

期刊名稱：JEM- J Exp Med

時間期卷：(2021) 218 (5): e20201452.

在線時間：2021年3月3日

DOI： doi.org/10.1084/jem.20201452

Liposoma巨噬細胞清除劑氯膦酸鹽脂質(zhì)體Clodronate Liposomes見刊于JEM：

Liposoma巨噬細胞清除劑氯膦酸鹽脂質(zhì)體Clodronate Liposomes的材料和方法：

JEM期刊率膦酸鹽脂質(zhì)體清除巨噬細胞專業(yè)論文：巨噬細胞清除解決方案。

Depletion of monocytes/macrophages and granulocytes

For depletion of granulocytes, mice were injected i.p. with 200 µg anti-Ly6G antibody or control antibody (Bio X Cell) 3 d before the first vincristine administration and then together with vincristine (i.p.; 0.5 mg/kg) injection as described previously (Daley et al., 2008; Starobova et al., 2019a). For depletion of monocytes, clodronate liposomes (10 µl/g of a 5-mg/ml solution) or control liposome-encapsulated PBS (Liposoma Research) were injected i.p. 3 d before the first vincristine administration and then together with vincristine (i.p.; 0.5 mg/kg) injection as described previously (Barclay et al., 2007; Old et al., 2014; Starobova et al., 2019a; Van Rooijen and Sanders, 1994). To confirm depletion of monocytes or granulocytes, the spleen or blood were collected after two doses of clodronate or Ly6G and/or 24 h after first vincristine (i.p.; 0.5 mg/kg) injection.